Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

Free eBooks at Library Online

Cinema Online,Free Movies-(1)

Progresses in Life Science

Free eBooks in biomedicine

Pathway databases

Biological Educational Resources

Textbooks and Lab Manuals

Yeast Protocols and Resource 

Yeast Culture and Storage

Yeast Cellular Biology

Yeast Genetics

Yeast Genome Database Links

Yeast Transformation

Two-Hybrid System

Yeast DNA and RNA Methods

Yeast PCR Protocols

Yeast Protein Methods

Yeast Histochemistry and Immunohistochemistry

Gene and protein sequence information

Other Yeast Protocols and Resource

Laboratory manuals, textbooks, references, and other Education Information

Other Yeast Protocols and Resource

 

Yeast Protocols Handbook

The World-Wide Web Virtual Library: Yeast

Yeast Sucrose Gradient Protocol

A microarray-based protocol for monitoring the growth of yeast ...

A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM)...

A Protocol Guide for the N. crassa Yeast Artificial Chromosome Library

American Type Culture Collection (ATCC) maintains yeast stocks and clones.

Common Access to Biological Resources and Information (CABRI) includes catalogs from European culture collections for yeast and other organisms.

Commonly used lab strains, a table providing genotypes, references, and other notes for some of the most commonly used lab strains.

Competent Yeast Preparation  

DNA Replication Protocols from the Fangman and Brewer Labs, University of Washington.

Find cloning vectors from NCBI's Taxonomy Browser.

Full text of the paper (Erdeniz et al. (1997) Genome Res. 7:1174-1183) describing PCR-Based Allele Replacement. Available to the public without password restriction and at no charge, through a special arrangement with Genome Research arranged by Rodney Rothstein.

Interaction Trap at Work information page from Erica Golemis's laboratory at the Fox Chase Cancer Center.

INTESTINAL DYSBIOSIS / YEAST PROTOCOL

Methods from the Fangman/Brewer Lab

Methods from the Gietz Lab

National Collection of Yeast Cultures (NCYC) maintains over 3,100 non-pathogenic yeasts, including type strains, strains of general interest for education and research, strains of industrial importance, and genetically marked strains.

Protocol for Yeast-Based Androgen Receptor Assay ...

Slide Stripping Protocol Agilent Yeast Arrays

The Definitive Yeast Transformation Homepage from the University ofManitoba.

The MGuide. Version 2.0, a complete guide to microarraying for the molecular biologist, from the Brown Lab, Stanford University.

The NCRR Yeast Resource Center at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction.

Tritech Research maintains a list of links to S. cerevisiae information and protocols, and sells reagents and equipment for S. cerevisiae research.

VectorDB, collected sequences of cloning vectors for use in a variety of organisms.

Yeast Actin Capture Assay  

Yeast Cell Micromanipulation, a protocol for making a support rod with fiber optic needle attached.

Yeast GST-fusion Collection developed in Eric Phizicky's laboratory. A total of 6080 individual yeast strains, each containing a plasmid expressing a different full-length yeast Open Reading Frame (ORF) fused to a glutathione S-transferase (GST). Hosted by ResGen Invitrogen.

Yeast IF without Dehydration

Yeast Knockout (YKO) Collection from the Saccharomyces Genome Deletion Consortium. Submit a comment to the Community Posting page. Read all comments.

Yeast Lab Protocols from the Botstein Lab, Stanford University.

Yeast Lab Protocols from the Breeden Lab, Fred Hutchinson Cancer Research Center.

Yeast Lab Protocols from the Gottschling Lab, Fred Hutchinson Cancer Research Center.

Yeast ProtoArrayTM Proteome Chips, containing 4,088 Saccharomyces cerevisiae open reading frames (ORFs) expressed as 5'-GST fusions from Invitrogen.

Yeast Protocols

Yeast Protocols from the Gottschling Lab

Yeast Sporulation    

Yeast Tet-promoters Hughes Collection containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by Open Biosystems.

Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, from the Yale Genome Analysis Center.

Yeast Two-Hybrid Analisys at the University of Washington's Yeast Resource Center. The entire set of Saccharomyces cerevisiae open reading frames (ORFs) fused to the Gal4 activation domain is available as an array of ~6000 transformants.

Yeast-GFP Clone Collection from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs). Hosted by Invitrogen.

Yeast-TAP Fusion Library from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations. Hosted by Open Biosystems.

 

Laboratory manuals, textbooks, references, and other Education Information

 

American Society for Microbiology. The ASM website lists resources in its Education section. The ASM publishes numerous books on microbiology education at various levels.

Discovering Genomics, Proteomics, and Bioinformatics This website describes a course and the accompanying textbook written by A. Malcolm Campbell and Laurie J. Heyer.

Genetics Education Network. Tom Manney's (Kansas State University) yeast experiments for undergrads and high school students. A well-done and informative web site for yeast in the classroom.

Genome Consortium for Active Teaching (GCAT) Description of the consortium and how it is working to bring functional genomics methods into the undergraduate biology curriculum.

MendelWeb, an educational resource on the origins of classical genetics.

Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, a series of laboratory manuals published by CSHL Press.

MicroArray Genome Imaging and Clustering Tool (MAGIC) Open source software for analysis of large-scale gene expression datasets; developed by Laurie Heyer and her undergraduate students at Davidson College, North Carolina.

National Association of Biology Teachers. High school teachers predominate in this organization, but there are resources for post-secondary education as well.

National Science Teachers Association. Resources for all science teachers from kindergarten to college.

NCBI Bookshelf, a growing collection of biomedical books that can be searched directly online.

Research Link 2000. Aimed at undergraduate classrooms, this is an offshoot of Tom Manney's yeast experiments. It also lists other model organisms.

The Molecular and Cellular Biology of the Yeast Saccharomyces, a monograph series containing comprehensive reviews about the biology of the yeast Saccharomyces cerevisiae published by CSHL Press.

Using Yeast As An Ultraviolet Light Measurement Tool. This is a Tom Manney protocol rewritten by Kevin

 

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