Laboratory Manual for Biomedical Research

 

New Edition

 

Chapter 6. Molecular Biology ¨C Protein

 

Protein methods

Protein labeling techniques

Protein sequencing

Subcellular fractionations

Western blot hybridization

Western Blot Hybridization Center

Western Blot Full Procedure (from Pierce)

Western Blotting Procedure with Video Show

Protocol 1: Western Blot

Sample protein preparation

Protein concentration assay

Electrophoresis and blotting

Blocking non-specific antigen

Incubation with primary antibody

Incubation with secondary antibody

Substrate (ECL) incubation

Restore a western blot using pierce stripping buffer

Western blot related solutions and buffers

Western blot handbook

Western blot troubleshooting

Protocol 2: Western Blot 2.

Protocol 3: Western Blot 3.

More online Western blot protocols (1) (2)

Protein chips

Kinase assay

Methods for detecting protein phosphorylation

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

Free eBooks at Library Online

Cinema Online,Free Movies-(1)

Progresses in Life Science

Free eBooks in biomedicine

Pathway databases

Biological Educational Resources

Textbooks and Lab Manuals

Lab-Manual.Com
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