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Second Edition


Chapter 1. General Lab Techniques


Lab security and basic techniques

Advanced lab skills

Advanced lab skills (2)


Chapter 2. Molecular Biology -- Molecular Separation & Labeling


Molecular Separation

Molecular Tracer Labeling

Chapter 3. Molecular Biology -- DNA and RNA


Nucleic acid methods (1)

Nucleic acid methods (2)

DNA isolation & related protocols

DNA Purification (glass milk vs electroelution)

DNA, RNA Sequencing

Recombinant DNA Techniques

RNA Isolation and Purification

Isolation of DNA,RNA, and Protein simultaneously.

DNA mutation detection by SSCP

Preparation of DNA and RNA probes

Southern blot hybridization

Northern blot hybridization

Loss of Heterozygosity (LOH)

Gene knockout protocol

SiRNA gene knockout

Plasmid and its usefulness

DNA library construction

Microarray protocols.

Gene transfection

Transformation protocols

Gene therapy for cancer

Molecular cloning

Conditional gene transfection(Tet on/off)


Chapter 4. Genetics


Epigenetics protocols

Mutagenesis protocols

Single nucleotide polymorphisms (SNPs)


Chapter 5. Molecular Biology --PCR Serials


PCR,RT-PCR,Real time PCR etc.

PCR (General Procedure)

PCR Primer Design Tools


Real time PCR

More PCR Protocols Online

Video and Animation of PCR

Mouse Genotyping by PCR

PCR Based Molecular Cloning

Variants of PCR

PCR Troubleshootings


Chapter 6. Molecular Biology -- Protein


Protein methods

Enzyme Assay Protocols (1)   (2)  (3)

Protein labeling techniques

Protein sequencing

Dot Blot

Subcellular fractionations

Western blot hybridization

Protein chips

Kinase assay

Methods for detecting protein phosphorylation


Chapter 7. DNA Protein Interactions



ChIP assay

Filter Binding (1)  (2)

DNase Footprinting (1)  (2)

DMS Footprinting

Southwestern Blotting

Genetic analysis

X-ray crystallography

Methylation Interference

Mapping protien/DNA interaction by cross-linking


Chapter 8. Immunohistochemistry / immunology


Preparing silanized (+plus) slides

Histological fixation

Microwave histology 

Microscopy Techniques

Confocal microscope technique

Electron microscopy

HE staining

DAPI & PI nucleic acid stain

Hybridization in situ


Special cell & cell fraction stains

Antibody purification

Antibody storage and handling

Conjugation of monoclonal antibodies

Antigen retrieval

Immunoperoxidase staining techniques



Histotechnology--technical methods

FRQs for histochemistry

Laser Capture Microdissection


Cytokine Assays Protocols  (1),  (2)


Chapter 9. Cellular Biology


General cell culture protocols

Tissue culture techniques

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Blood cell fractionation (white blood cell isolation)

Endothelial cell isolation and culture protocols

Flow cytometry (FCM)


Chapter 10. Metabolism, GC/MS, NMR and Proteomics


GC/MS Background

Glucose metabolism and its related protocols

Glucose and Insulin Tolerance Test

PAS staining

Deoxyribose procedure

Ribose metabolism analysis

Polysaccharide sequencing

Lactate cleanup and derivative

Amino acids

Fatty acids

Lipid protocols


Bile acids

Urea procedure

Choline Incorporation Assay

Isotope Ratio Mass Spectrometer (IRMS)

Liquid chromatography / mass spectrometer (LC/MS)


NMR protocols and tutorials


Chapter 11. Animal Experiments


Blood sampling from animals

Basic skills for animal experiments

Anatomy and physiology of animals

Tail Vein Injection Techniques  (1)  (2)

Cancer xenograft animal models

Transgenic animal procedures

Transgenic cancer models

Murine models of human breast cancer

Animal models for depression-like and anxiety-like behavior


Chapter 12. Model Organisms 


C. Elegans protocols

Yeast protocols


Chapter 13. HPLC and TLC


HPLC protocols



Chapter 14. Buffers and Solutions in Lab.


Commonly Used Buffer and solution (114 formats in alphabeta)

Compound solutions (Practical Molecular Biology)
How to Make Simple Solutions and Dilutions (Department of Biology, Bates College)
Reto's Buffers&Media Book (Private Page)
Salt solutions (Practical Molecular Biology)
Single Component Solutions (Practical Molecular Biology)
Buffers (Gerard R. Lazo)

Antibiotics (Gerard R. Lazo)
Buffer Listing (Salmon Lab.)

Cell Culture Media and Solutions (Donis-Keller lab)

Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 )

Commonly used solutions (2)--- 1 ) 2 ) 3 )

Solutions for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )


Chapter 15. Other Resources


Free eBooks at Library Online

Online Guides in Life Science

Super Textbook of Biochemistry

Cinema Online, Free Movies --(1) (2) (3)

Progresses in Life Science

Progress in Cancer Apoptosis -- (1) (2) (3)

Progress in Gene Therapy --- (1) (2) (3)

Progress in Neuronal Regeneration -- (1) (2) (3)

Progress in AIDS Treatment -- (1) (2) (3)

Progress in Organ Transplantation -- (1)  (2)  (3)

Progress in Stem Cell Research -- (1)  (2)  (3)

Free eBooks in life science (1)

Free eBooks in life science (2,3)

Most Updated Biomedical Books

Pathway databases

Pathway Search

Biomedical Job Opportunities

Tools for Statistics

Free Software pDRAW32 to Draw DNA Analysis Charts

Biological Educational Resources

Genetics Education

Resources of Medical Biochemistry

Textbooks and Lab Manuals


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