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Second Edition

 

Chapter 1. General Lab Techniques

 

Lab security and basic techniques

Advanced lab skills

Advanced lab skills (2)

 

Chapter 2. Molecular Biology -- Molecular Separation & Labeling

 

Molecular Separation

Molecular Tracer Labeling

Chapter 3. Molecular Biology -- DNA and RNA

 

Nucleic acid methods (1)

Nucleic acid methods (2)

DNA isolation & related protocols

DNA Purification (glass milk vs electroelution)

DNA, RNA Sequencing

Recombinant DNA Techniques

RNA Isolation and Purification

Isolation of DNA,RNA, and Protein simultaneously.

DNA mutation detection by SSCP

Preparation of DNA and RNA probes

Southern blot hybridization

Northern blot hybridization

Loss of Heterozygosity (LOH)

Gene knockout protocol

SiRNA gene knockout

Plasmid and its usefulness

DNA library construction

Microarray protocols.

Gene transfection

Transformation protocols

Gene therapy for cancer

Molecular cloning

Conditional gene transfection(Tet on/off)

 

Chapter 4. Genetics

 

Epigenetics protocols

Mutagenesis protocols

Single nucleotide polymorphisms (SNPs)

 

Chapter 5. Molecular Biology --PCR Serials

 

PCR,RT-PCR,Real time PCR etc.

PCR (General Procedure)

PCR Primer Design Tools

RT-PCR

Real time PCR

More PCR Protocols Online

Video and Animation of PCR

Mouse Genotyping by PCR

PCR Based Molecular Cloning

Variants of PCR

PCR Troubleshootings

 

Chapter 6. Molecular Biology -- Protein

 

Protein methods

Enzyme Assay Protocols (1)   (2)  (3)

Protein labeling techniques

Protein sequencing

Dot Blot

Subcellular fractionations

Western blot hybridization

Protein chips

Kinase assay

Methods for detecting protein phosphorylation

 

Chapter 7. DNA Protein Interactions

 

EMSA

ChIP assay

Filter Binding (1)  (2)

DNase Footprinting (1)  (2)

DMS Footprinting

Southwestern Blotting

Genetic analysis

X-ray crystallography

Methylation Interference

Mapping protien/DNA interaction by cross-linking

 

Chapter 8. Immunohistochemistry / immunology

 

Preparing silanized (+plus) slides

Histological fixation

Microwave histology 

Microscopy Techniques

Confocal microscope technique

Electron microscopy

HE staining

DAPI & PI nucleic acid stain

Hybridization in situ

FISH

Special cell & cell fraction stains

Antibody purification

Antibody storage and handling

Conjugation of monoclonal antibodies

Antigen retrieval

Immunoperoxidase staining techniques

Immunofluorohistochemistry

Immunoprecipitation

Histotechnology--technical methods

FRQs for histochemistry

Laser Capture Microdissection

Elisa

Cytokine Assays Protocols  (1),  (2)

 

Chapter 9. Cellular Biology

 

General cell culture protocols

Tissue culture techniques

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Blood cell fractionation (white blood cell isolation)

Endothelial cell isolation and culture protocols

Flow cytometry (FCM)

 

Chapter 10. Metabolism, GC/MS, NMR and Proteomics

 

GC/MS Background

Glucose metabolism and its related protocols

Glucose and Insulin Tolerance Test

PAS staining

Deoxyribose procedure

Ribose metabolism analysis

Polysaccharide sequencing

Lactate cleanup and derivative

Amino acids

Fatty acids

Lipid protocols

Cholesterol

Bile acids

Urea procedure

Choline Incorporation Assay

Isotope Ratio Mass Spectrometer (IRMS)

Liquid chromatography / mass spectrometer (LC/MS)

Proteomics

NMR protocols and tutorials

 

Chapter 11. Animal Experiments

 

Blood sampling from animals

Basic skills for animal experiments

Anatomy and physiology of animals

Tail Vein Injection Techniques  (1)  (2)

Cancer xenograft animal models

Transgenic animal procedures

Transgenic cancer models

Murine models of human breast cancer

Animal models for depression-like and anxiety-like behavior

 

Chapter 12. Model Organisms 

 

C. Elegans protocols

Yeast protocols

 

Chapter 13. HPLC and TLC

 

HPLC protocols

TLC

 

Chapter 14. Buffers and Solutions in Lab.

 

Commonly Used Buffer and solution (114 formats in alphabeta)

Compound solutions (Practical Molecular Biology)
How to Make Simple Solutions and Dilutions (Department of Biology, Bates College)
Reto's Buffers&Media Book (Private Page)
Salt solutions (Practical Molecular Biology)
Single Component Solutions (Practical Molecular Biology)
Buffers (Gerard R. Lazo)

Antibiotics (Gerard R. Lazo)
Buffer Listing (Salmon Lab.)

Cell Culture Media and Solutions (Donis-Keller lab)

Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 )

Commonly used solutions (2)--- 1 ) 2 ) 3 )

Solutions for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )

 

Chapter 15. Other Resources

 

Free eBooks at Library Online

Online Guides in Life Science

Super Textbook of Biochemistry

Cinema Online, Free Movies --(1) (2) (3)

Progresses in Life Science

Progress in Cancer Apoptosis -- (1) (2) (3)

Progress in Gene Therapy --- (1) (2) (3)

Progress in Neuronal Regeneration -- (1) (2) (3)

Progress in AIDS Treatment -- (1) (2) (3)

Progress in Organ Transplantation -- (1)  (2)  (3)

Progress in Stem Cell Research -- (1)  (2)  (3)

Free eBooks in life science (1)

Free eBooks in life science (2,3)

Most Updated Biomedical Books

Pathway databases

Pathway Search

Biomedical Job Opportunities

Tools for Statistics

Free Software pDRAW32 to Draw DNA Analysis Charts

Biological Educational Resources

Genetics Education

Resources of Medical Biochemistry

Textbooks and Lab Manuals

 

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