BioProtocols--western

Protocol 1: Full Procedure for Western Blot

1) Sample protein preparation

2) Protein concentration assay

3) Electrophoresis and blotting

4)Blocking non-specific antigen

5) Incubation with primary antibody

6) Incubation with secondary antibody

7)Substrate (ECL) incubation

Restore a western blot using pierce stripping buffer

Western blot troubleshooting

Protocol 2: Western Blot 2.

Protocol 3: Western Blot 3.

Protocol 4: Modified Protocol for Western Blotting (Page 1, 2, 3)

Western blot for phosphorylated proteins

Western blot handbook

Western Blot World -- From Wikipedia, the free encyclopedia

Contents

1 Steps in a western blot

1.1 Tissue preparation

1.2 Gel electrophoresis

1.3 Transfer

1.4 Blotting

1.5 Detection

1.5.1 Two step

1.5.2 One step

1.6 Analysis

1.6.1 Colorimetric detection

1.6.2 Chemiluminescence

1.6.3 Radioactive detection

1.6.4 Fluorescent detection

1.7 Secondary probing

2 2-D Gel Electrophoresis

3 Medical diagnostic applications

4 Protocols

5 References

6 See also

6.1 Related links

More online Western blot protocols (1) (2)