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Biotechniques - Your lab's handbook
C. Elegans: Embryonic cell culture

Studying cellular processes in a living C. elegans offers the advantage of a physiological environment in which these processes occur. However, because of the small size of the animal, and because the cuticle of the animal is a significant permeability barrier, many experiments are difficult or impossible to perform. Thus, for example,

 
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions Print E-mail
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Sunday, 10 April 2011 15:24

Introduction: Human embryonic stem cells (hESCs) have the potential to differentiate into all three germ layers and proliferate in long-term culture in vitro. hESCs can provide a cell source for the testing of novel therapies, drug screening, and functional genomics applications. Undifferentiated hESCs can be maintained and proliferated on mouse embryonic

Last Updated on Monday, 03 November 2014 16:49
 
Polar Lipid Extraction Print E-mail
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Monday, 29 August 2011 12:33

 

Polar lipids are generally extracted from dry cell material using chloroform:methanol:0.3% NaCl (1:2:0.8 v/v/v). This may be carried out by adding 9.5 ml of this mixture to 100 mg of freeze dried cells, or by adding a suitable amount of chloroform,methanol and 0.3% NaCl to the cell material, or to the aqueous methanolic phase remaining from the

 
Genetic Analyses

Understanding the underlying structural and physico-chemical basis for the recognition of specific DNA sequences by regulatory proteins is a central goal of modern biochemical genetics. A method for the rapid identification of mutant molecules altered in the affinity and/or specificity of such interactions could be a powerful tool in the hands of those studying this difficult problem. 

 
H and E Stain Protocols
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Friday, 26 September 2008 02:30

H&E stain, HE stain or hematoxylin and eosin stain, is a popular staining method in histology. It is the most widely used stain in medical diagnosis; for example when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H&E and termed H&E section, H+E section, or HE section.

Last Updated on Saturday, 01 November 2014 13:41
 

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