Microarray Protocols
 
Basic knowledge of microarray.
 
Basic knowledge of microarray 2.
 
Introduction to Microarray.
 
A Mediabook of Microarrays
 
Protein chips 
 
MicroArray Procedure

 

(Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall, 8.20.2000)

 

1. Total RNA Isolation from cultured cells.

 

2. DNase Treatment of Total RNA 

 

3. Making the single strand cDNA probe.

 

4. Automated Slide Processor (ASP) Version for hybridization.

 

5. Washing microarrays in ASP.

 

6.Processing of Array slide

 

7. Pre-hybridization of the processed slides (NON-Automated version).

 

8. Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).

 

9. Preparation of Dendrimer Cy3 and Cy5.

 

10. Washing unbound probe from glass array (NON-Automated version).

 

11. Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).

 

12.Washing unbound dendrimer from glass array(NON-Automated version).

 

Microarray Dababases

 

Troubleshooting

 

Other Microarray Protocols (1)

 

Other Microarray Protocols (2)
 
Other Microarray Protocols (3)
 
                                                                                            
 
 
 

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

Free eBooks at Library Online

Cinema Online,Free Movies-(1)

Progresses in Life Science

Free eBooks in biomedicine

Pathway databases

Biological Educational Resources

Textbooks and Lab Manuals

Lab-Manual.Com
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