Laboratory Manual for Biomedical Research

 

New Edition

 

Chapter 9. Cellular Biology

 

General cell culture protocols

Tissue culture techniques

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Introduction

What are the unique properties of all stem cells?

What are embryonic stem cells?

What are adult stem cells?

What are the similarities and differences between embryonic and adult stem cells?

What are the potential uses of human stem cells and the obstacles that must be  overcome before these potential uses will be realized?

Where can I get more information?

International Society for Stem Cell Research (ISSCR)

Culture of Human Embryonic Stem Cells (hESC)

Establishment and characterization of human embryonic stem cell line

Rat stem cell isolation and culture

Mouse stem cell isolation and culture

Induction of Stem Cell Differentiation

Cancer stem cells

Video Data on Stem Cell Research (click to show)

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Blood cell fractionation (white blood cell isolation)

Endothelial cell isolation and culture protocols

Flow cytometry (FCM)

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

Free eBooks at Library Online

Cinema Online,Free Movies-(1)

Progresses in Life Science

Free eBooks in biomedicine

Pathway databases

Biological Educational Resources

Textbooks and Lab Manuals

Lab-Manual.Com
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