Laboratory Manual for Biomedical Research
New Edition
Chapter 3. Molecular Biology -- DNA and RNA
DNA isolation & related protocols
DNA Purification (glass milk vs electroelution)
RNA Isolation and Purification
Isolation of DNA,RNA, and Protein simultaneously.
DNA mutation detection by SSCP
Preparation
of DNA and RNA probes
Basic knowledge of microarray.
MicroArray Procedure
Total RNA Isolation from cultured cells.
Making the single strand
cDNA probe.
Automated Slide Processor (ASP) Version for hybridization.
Pre-hybridization
of the processed slides (NON-Automated version).
Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).
Preparation
of Dendrimer Cy3 and Cy5.
Washing unbound probe from glass array (NON-Automated version).
Hybridization of Dendrimers (Cy3 and Cy5)
to Array (NON-Automated version).
Washing unbound dendrimer from glass array(NON-Automated version).
Other
Microarray Protocols (1,2)
Conditional gene transfection(Tet
on/off)
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
