Yeast Protocols and Resource 

Yeast Culture and Storage

Yeast Cellular Biology

Yeast Genetics

Yeast Genome Database Links

Yeast Transformation

Two-Hybrid System

Yeast DNA and RNA Methods

Yeast PCR Protocols

Yeast Protein Methods

Yeast Histochemistry and Immunohistochemistry

Gene and protein sequence information

Other Yeast Protocols and Resource

Laboratory manuals, textbooks, references, and other Education Information

Streaking yeast stocks (Donis-Keller lab)

Long term storage of yeast stocks (Donis-Keller lab)

Media & Solutions & Stocks (Donis-Keller lab)

Sporulation & Dissection (Hahn Lab)

Freezedown guidelines (Saccharomyces Genome Deletion Project)

Liquid sporulation (Herskowitz Lab)

Old yeast cell sorting protocol (Sinclair Lab)

YPD filled microtiter plates (Saccharomyces Genome Deletion Project)

Candida methods (University of Minnesota)

Yeast protocols handbook (CLONTECH, pdf)

Preparation of yeast cells for immuno-electron microscopy(BioVisa.net, pdf)

IF with methanol/acetone dehydration (Botstein Lab)

Cell cycle by flow cytometry (Salk Institute)

FACS analysis (Sinclair Lab)

Extraction and purification of ascorbic acid analogs in biological samples using HPLC (BRIC)

Preparation of gel samples for pulsed-field gel electrophoresis (BRIC)

Purification of D-arabinono-1,4-lactone oxidase from the mitochondrial fraction (BRIC)

MET15 Resource center (lead plates)

YC Dropout Plate Supplementation Table

YC media

Yeast Media  

Yeast Cellular Biology

Colony morphology

Dapi Staining

DAPI Staining of Yeast

FACS Analysis

Flow Cytometry of yeast   

Immunofluorescence for Epitope Tagged Proteins

INVITTOX PROTOCOL no.33 YEAST GROWTH RATE CYTOTOXICITY TEST

Yeast Cell Cycle Flow Cytometry Protocol

Yeast FACS Analysis

Yeast FACS protocol