DNA Isolation and Related Protocols

 

Contents

 

 

Genome DNA Extraction

 

DNA Isolation Procedures  (totally 22 protocols for DNA isolation)

Protocol for Isolation of Genomic DNA from Mammalian Cells

Protocol for Isolation of Genomic DNA from Mammalian Cells (with RNase A, DNase and protease-free). Trypsinize, harvest and resuspend cells at 107/ ml in 10 ...
www.fermentas.com/techinfo/modifyingenzymes/protocols/p_isolgendna.htm -

Genomic DNA Purification
Genomic DNA Purification. 13. Figure 5. DNA was purified from tissue samples. pooled from 10 mice using the DNeasy Tissue protocol. for rodent tails. ...
www1.qiagen.com/literature/brochures/Gen_DNA_Pur/1019469_BROS_DNYTi_

GenElute Mammalian Genomic DNA Miniprep Kit - Purification of ...

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of ...
www.sigmaaldrich.com/.../DNA_and_RNA_Purification/GenElute_Mammalian_

III. Methods for DNA isolation

Genomic DNA isolation is performed according to the FBI protocol (27). After the blood samples (stored at -70degC in EDTA vacutainer tubes ) are thawed, ...
www.genome.ou.edu/protocol_book/protocol_partIII.html

Genomic DNA Purification Protocol

Genomic DNA Purification Protocol. Modification to the salting out procedure of Miller, S.A., et al., Nucleic Acids Research, 16, 1215 (1988). ...
wheat.pw.usda.gov/~lazo/methods/lazo/met9.html 

DNA Extraction Protocol 1

Concentration of original DNA solution in ?g/ml = Abs x 100 x 50 ?g/ml. Protocol of Genomic DNA Isolation from a Gram-Negative Bacterium ...
www.hawaii.edu/microbiology/MO/dnaextract1.htm

Genomic DNA Extraction Protocol

DNA Extraction from Cell and Tissue. Lysis Buffer:. 50 mM Tris-HCl, pH 8.0 50mM EDTA ... Make sure DNA is completely dissolved (leave on gentle shaker.) ...
www.komabiotech.co.kr/technical/protocol/DNAexcell.htm

DNA Isolation

Isolation of Genomic Dna from Mouse Tail or Spleen (after Laird et al., 1991). Overview ? Lysis (steps 1-2) ? Isopropanol precipitation (3-4) ...
www.nervenet.org/papers/DNAIsolation.html

Genomic DNA Isolation Protocol - MiniBeadbeater 1.
Genomic DNA Isolation Protocol - MiniBeadbeater. 1. Label 1 screw-capped tube and 4 microfuge tubes (1.5 ml) for each sample to be used ...
www.csun.edu/~mls42367/Protocols/MiniBeadbeater%20DNA%20isol.pdf -

Protocol for Isolation of Genomic DNA from Blood, Cultured Cells ...
Genomic-tip Protocol. QIAGEN Genomic DNA Handbook 08/2001. 44. Part II: Genomic-tip Protocol. Protocol for Isolation of Genomic DNA from Blood, ...
molecool.wustl.edu/.../Molecular%20Biology%20protocols/Genomic%20DNA%20isolation/Genomic-tip%20protocol.pdf -

Molecular Biology Laboratory DNA Isolation Manual

Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM. (Modified from Experimental Techniques in Bacterial Genetics, Jones and Bartlet, 1990) ...
www.research.umbc.edu/~jwolf/m1.htm

NucPrep Chemistry: Isolation of Genomic DNA from Animal and Plant ...
Chemistry: Isolation of Genomic DNA from Animal and Plant Tissue. Time Required to. Complete This. Protocol. By using Applied Biosystems NucPrep chemistry, ...
docs.appliedbiosystems.com/pebiodocs/04333959.pdf

JETFLEX Genomic DNA Purification Handbook

GENOMED?s JETFLEX Genomic DNA Purification Kit provides all reagents necessary to prepare .... c) Re-purification protocol for still impure DNA?s ...
www.genomed-dna.com/pdf/Flex-PDFs/JETFLEX_Protocol%20(updated).PDF -

Huberman DNA prep

Joel Huberman's DNA isolation procedure with modifications by Bonny Brewer. 1. Kill cells at mid log phase by adding Na Azide to 0.1%. ...
fangman-brewer.genetics.washington.edu/DNA_prep.html

 
 

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

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Pathway databases

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