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Molecular Biology / PCR / Variants of PCR / PCR SSCP
Protocol: Mutation Detection by SSCP PCR.
A protocol for mutation detection
by single-strand conformational polymorphism (SSCP) by PCR from the neurogenetics laboratory in the Neurological Sciences ...
www.ohsu.edu/nsi/faculty/reddyh/lab/protsscp.html
Step by Step SSCP. Travis Glenn. Laboratory of Molecular Systematics. Smithsonian Institution.
www.uga.edu/srel/DNA_Lab/SSCP'96V2.rtf
Springer Protocols is the largest subscription-based
electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.
www.springerprotocols.com/Abstract/doi/10.1385/0-89603-499-2:51
PCR-SSCP:
a practical approach (detailed SSCP protocols)
The multiplexed PCR-SSCP analysis described here (Protocol 5) is essentially a two step
procedure, each strand of the target DNA sequence is labelled as it ...
europium.csc.mrc.ac.uk/WebPages/Database/Methods/pcrpract.htm
Optimization of Nonisotopic PCR¨CSingle-Strand Conformation Polymorphism.
The protocol used for the GCK gene allowed us to establish
a successful strategy for the development of PCR-SSCP on other genes such as BRCA1 (breast cancer ...
www.clinchem.org/cgi/content/full/43/11/2190
Genomic
Variation Laboratory ¨C SSCP Protocol
Feb 27, 2003 ... SSCP Protocol. MDE gel (BioWhittaker Molecular Applications) final ... 3) For
single strands, mix 2 ul PCR product for each sample with 10 ...
http://genome-lab.ucdavis.edu/Protocols/SSCP%20Protocols.htm
Evaluating
Duplicate Gene Expression using RT-PCR/SSCP Analysis (Wendel Lab.)
The subsequent PCR reaction follows the protocol you have predetermined
to work best .... 1995, Identification of DNA polymorphism by asymmetric-PCR SSCP. ...
http://www.eeob.iastate.edu/faculty/WendelJ/rt-pcr_sscp.htm
Sensitive
detection of p53 gene mutations by a 'mutant enriched PCR SSCP technique.
In the past, the existing PCR-SSCP technique as established
by Orita et al. ... Figure 1 gives a schematic view of the protocol applied whereby the ...
nar.oxfordjournals.org/cgi/content/full/26/5/1356
Single-strand conformational polymorphism.
cantly to its utility. SSCP PROTOCOL. The following is an example of an SSCP protocol
that we used for detection. $138 PCR Methods and Applications ...
www.genome.org/cgi/reprint/4/3/S137.pdf?ck=nck