Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

Free eBooks at Library Online

Cinema Online,Free Movies-(1)

Progresses in Life Science

Free eBooks in biomedicine

Pathway databases

Biological Educational Resources

Textbooks and Lab Manuals

Molecular Biology / PCR / Variants of PCR / PCR RFLP

 

Restriction fragment length polymorphism (From Wikipedia, the free encyclopedia):

A Restriction Fragment Length Polymorphism (or RFLP, often pronounced as "rif-lip") is a variation in the DNA sequence of a genome which can be detected by a laboratory technique known as gel electrophoresis. Analysis of RFLP variation was an important tool in genome mapping, localization of genetic disease genes, determination of risk for a disease, genetic fingerprinting, and paternity testing.

 

Contents

1 Analysis technique

2 Examples

3 Applications

4 Alternatives

5 References

5.1 External links

 

PCR-RFLP Method

18-Oct-02.
www.ihwg.org/components/cytokine/mon8Lin.htm

 

Examples of PCR-RFLP for Nematode Diagnostics

Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.
nematode.unl.edu/its_id/EXAMPLES/index.htm

 

Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences

Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences.
www.kfunigraz.ac.at/~binder/thesis/node64.html

 

RFLP/PCR Polymorphism Query Form

Search for RFLP and PCR based polymorphisms by strain, locus symbol, or map position. To search for SNPS, use the SNP Query Form. ...
www.informatics.jax.org/searches/polymorphism_form.shtml

 

PCR, RFLP and Gene Therapy

Lecture 24:Genetic Engineering: PCR, RFLP Analysis & Gene Therapy. The Polymerase Chain Reaction (PCR) Can Make Millions of Copies of DNA in a Short Time ...
http://members.aol.com/BearFlag45/Biology1A/LectureNotes/lec24.html

 

Handbook for DNA isolation, RAPD-PCR and PCR-RFLP

General protocol. We use agarose gels for checking the quality of DNA isolates, PCR products, and PCR-RFLP products, and for scoring RAPD products. ...
www.toyen.uio.no/botanisk/brochmann/handbook.htm

 

PCR-RFLP PROTOCOL FOR ALLELES A AND B PCR ...
BOVINE KAPPA-CASEIN PCR-RFLP PROTOCOL. FOR ALLELES A AND B. Laboratory of J.F. Medrano. Department of Animal Science. University of California. ...
animalscience.ucdavis.edu/laboratory/

 

Lab-Manual.Com
Home || Biosocial || Videos || Recipes || Resource