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Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
Molecular Biology / PCR / Variants of PCR / RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where
the target sequence(s) (to be amplified) is unknown.Brief description, from
avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html
Type, Initialize, X, Denature, Anneal, Extend, Termination. RAPD 10-mer, 95C, 5min, 45, 94C, 0:30, 35C,
1:00, 72C, 2:00, 72C, 5:00. PCR 18+mer, 95C, 5:00 ...
wheat.pw.usda.gov/~lazo/methods/lazo/pcrmeth0.html
Handbook for DNA isolation,
RAPD-PCR and PCR-RFLP
A standard RAPD-PCR reaction is performed in volumes of 25.0 m l containing 50 mM KCl, 10 mM Tris-HCl pH 9.0,
1.5 mM MgCl2, 0.001% gelatine, ...
www.toyen.uio.no/botanisk/brochmann/handbook.htm
RAPD Protocol dNTP mix (5mM): Loading buffer
(6X): Ladder mix: PCR .
RAPD Protocol. Protocol for Promega. and Stoffel brand Taq. enzymes (1 reaction):. 4 ¦Ěl dNTP (5mM) mix. 2.5
¦Ěl 10X PCR buffer. 8.33 ¦Ěl H ...
www.css.msu.edu/bean/PDF/RAPD_protocol.pdf
PCR PROTOCOL FOR RAPDs--1
PCR PROTOCOL FOR RAPDs.
Application: Fingerprinting, molecular mapping, ... Amount of DNA needed per RAPD reaction according to the plant species under ...
gene4.ciat.cgiar.org/limsys3.0/Modelos/protocolos/PCRPROTOCOLFORRAPDs.doc