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Molecular Biology / PCR / Variants of PCR / Colony PCR Protocols
Detailed protocol from the web site
of the Department of Biology,
www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/colony_pcr.html
Colony
PCR
Colony PCR. This protocol is designed to quickly screen for plasmid inserts directly from E. coli. colonies. The plasmid should
be high copy number such as ...
www.csun.edu/~mls42367/Protocols/Colony%20PCR.pdf
Colony PCR Protocol
Colony PCR Protocol. 1. Pull
out eight glycerol stock plates from the ¨C80. o. C freezer and set on bench top to. thaw. Be sure to remove the foil seal ...
microarrays.berkeley.edu/file_download.php?fid=13
Colony
PCR Protocol
Colony PCR Protocol. Overnight Culture 5.0¦Ìl. 10x PCR buffer 5.0¦Ìl. MgCl2 (25mM) 5.0¦Ìl. Forward primer (10¦ÌM) 0.2¦Ìl.
Reverse primer (10¦ÌM) 0.2¦Ìl ...
www.unc.edu/~fconlon/Protocols/Colony%20PCR%20Protocol.doc
Colony PCR Protocol contributed
by Lynn Hancock 1. Choose a reasonable sized colony (2-3 mm in diameter) and resuspend 100 ¦Ìl ddH2O. 2. For PCR, ...
www.enterococcus.ouhsc.edu/ColonyPCRProtocol.asp
Yeast
Colony PCR -- Amberg et al.
Search CSH Protocols. Advanced Search ¡¤ Find Protocols ¡¤ Find a Kit ¡¤ Access Personal Page ¡¤ Submit
Protocols ¡¤ Help ¡¤ Subscribe ¡¤ About CSH Protocols ...
www.cshprotocols.org/cgi/content/short/2006/1/pdb.prot4170
From OpenWetWare. Jump to: navigation, search. See Colony PCR for general information about this protocol and other variants
...
openwetware.org/wiki/Endy:Colony_PCR
Monserate Biotechnology Group Custom services, Colony PCR protocol.
www.monseratebiotech.com/colony-pcr.html
Yeast
Colony PCR protocol
Yeast Colony PCR protocol. derived from http://sequence-. www.stanford.edu/group/yeast_deletion_project/deletions3.html.
Colony PCR (Forsburg Lab)
The Forsburg lab pages: fission yeast colony PCR protocol. ... Colony PCR can be used to identify colonies
where your favorite gene yfg1 has been replaced ...
http://www-rcf.usc.edu/~forsburg/pcr.html
Rapid Screening by Direct Colony
PCR Using the FastStart PCR Master
The FastStart PCR Master is a convenient and ideal tool for direct colony PCR. Intact bacteria can
be analyzed directly without prior template purification, ...
www.analytica-world.com/articles/e/65346/