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Molecular Biology / PCR / Variants of PCR / AFLP PCR
What¡¯s AFLP?-- From Wikipedia, the free encyclopedia
Amplified
fragment length polymorphism PCR (or AFLP-PCR or just AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and
in the practice of genetic engineering. Developed in the early 1990¡¯s by Keygene, AFLP uses restriction enzymes to cut genomic DNA,
followed by ligation of complementary double stranded adaptors to the ends of the restriction fragments. A subset of the restriction
fragments are then amplified using two primers complementary to the adaptor and restriction site fragments. The fragments are visualized
on denaturing polyacrylamide gels either through autoradiography or fluorescence methodologies.
AFLP-PCR is a highly sensitive
method for detecting polymorphisms in DNA. The technique was originally described by Vos and Zabeau in 1993. The procedure of this
technique is divided into three steps:
1. Digestion of total cellular DNA with one or more restriction enzymes and ligation
of restriction half-site specific adaptors to all restriction fragments.
2. Selective amplification of some of these fragments
with two PCR primers that have corresponding adaptor and restriction site specific sequences.
3. Electrophoretic separation of
amplicons on a gel matrix, followed by visualisation of the band pattern.
A variation on AFLP is cDNA-AFLP, which is used to quantify
differences in gene expression levels.
Another variation on AFLP is TE Display, used to detect transposable element mobility.
AFLP
protocol
AFLP protocol
AFLP Technologies
AFLP: principles and applications
AFLPs on the ABI 3100
Amplified Fragment Length Polymorphism
Protocol
Standard List for AFLP Primer Nomenclature
AFLP: not only for fingerprinting, but for positional cloning
AFLP PCR
In silico
AFLP-PCR amplification
AFLP: not only for Fingerprinting, but for Positional Cloning
GE Healthcare Life Sciences - AFLP
AFLP (Liscum
Lab.)
A PCR-based DNA fingerprinting technique: AFLP.
References:
AFLP Protocol Using Li-Cor Sequencer
http://www.oznet.ksu.edu/wheatgenotyping/aflp.html
Selected links about AFLP.
... Amplified Fragment Length Polymorphisms and Microsatellites: A phylogenetic perspective This paper reviews the techniques of ...
www.pcrlinks.com/variants/aflp.htm
Amplified
Fragment Length Polymorphism | World of Genetics
Amplified Fragment Length Polymorphism | World of Genetics. Amplified Fragment Length
Polymorphism summary with 1 pages of encyclopedia entries, ...
www.bookrags.com/.../amplified-fragment-length-polymorph-wog/
Fluorescent
Amplified Fragment Length Polymorphism Analysis of ...
Fluorescent Amplified Fragment Length Polymorphism Analysis of Norwegian Bacillus
cereus and Bacillus thuringiensis Soil Isolates. Ticknor LO,
www.ncbi.nlm.nih.gov/pubmed/11571195
Fluorescent
amplified fragment length polymorphism analysis of ...
Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis,
Bacillus cereus, and Bacillus thuringiensis isolates. ...
www.ncbi.nlm.nih.gov/pubmed/14766590
Google Directory - Science
> Biology > Biochemistry and Molecular ...
A description of the Amplified Fragment Length Polymorphism technique of DNA fingerprinting.
By U Melcher,
www.google.com/.../Science/.../Biochemistry_and_Molecular_Biology/.../PCR/....
Genetic
Analysis - Amplified Fragment Length Polymorphism
Amplified Fragment Length Polymorphism (AFLP) analysis is one of the most powerful
DNA fingerprinting methodologies for the identification and typing of ...
www.beckmancoulter.com/.../applications/.../ceq_aflp_app_stat_dcr.asp...
Amplified
fragment length polymorphism based identification of ...
Amplified fragment length polymorphism based identification of genetic markers
and novel PCR assay for differentiation of Campylobacter fetus subspecies ...
jmm.sgmjournals.org/cgi/content/abstract/54/12/1217
Impact
of Amplified Fragment Length Polymorphism Size Homoplasy on ...
Impact of Amplified Fragment Length Polymorphism Size Homoplasy on
the Estimation of Population Genetic Diversity and the Detection of Selective Loci ...
www.genetics.org/cgi/content/abstract/179/1/539
A
Combined Amplified Fragment Length Polymorphism and Randomly ...
(RAPD) and amplified fragment length polymorphisms. and other tree
species (Crous 1998). Septoria tritici. (AFLPs) allows a highly efficient generation of ...
www.genetics.org/cgi/reprint/161/4/1497.pdf
Fluorescence-labeled
Methylation-sensitive Amplified Fragment ...
Fluorescence-labeled Methylation-sensitive Amplified Fragment Length Polymorphism (FL-MS-AFLP)
Analysis for Quantitative Determination of DNA Methylation ...
jjco.oxfordjournals.org/cgi/content/abstract/38/4/317
Amplified
fragment length polymorphism analysis of different ...
eral utility of amplified fragment length polymorphisms. (AFLPs) for molecular
analysis of insect genomes. For highly. complex genomes, such as those from ...
journals.cambridge.org/.../action/cjoGetFulltext%3Ffulltextid%3D889168...
Amplified
Fragment Length Polymorphisms (AFLPs) detected with non ...
anthracis as detected by Amplified fragment length polymorphism markers.
J. Bact. 179:818
pubs.nrc-cnrc.gc.ca/ispmb/ispmb15/15255-3.pdf
K-State Research Exchange: Amplified fragment length polymorphism
...
Title: Amplified fragment length polymorphism in Mycosphaerella graminicola. Authors: Kabbage, Mehdi. Graduation Date: May 2007.
Type: Dissertation ...
krex.k-state.edu/dspace/handle/2097/255 [cache]
Amplified-Fragment Length Polymorphism Fingerprinting
of ...
Amplified-fragment length polymorphism (AFLP) is a whole-genome fingerprinting method based on selective amplification of restriction
fragments. ...
jcm.asm.org/cgi/content/abstract/37/10/3300
Using Amplified Fragment Length Polymorphisms and Simple Sequence
...
We have used two DNA-based methods, amplified fragment length polymorphisms (AFLPs) and simple sequence length polymorphisms (SSLPs),
to distinguish six ...
crop.scijournals.org/cgi/content/abstract/39/6/1715
Amplified fragment length polymorphisms and sequence
data in the ...
4 Phenogram of the neighbor-joining analysis of amplified fragment length polymorphism (AFLP) fingerprints using Nei
www.blackwell-synergy.com/.../pdf/.../j.1469-8137.2007.02172.x
Brazilian
Journal of Microbiology - Use of single-enzyme amplified ...
Use of single-enzyme amplified fragment length polymorphism for typing
Clostridium ... In the present study amplified fragment length polymorphism analyses ...
www.scielo.br/scielo.php%3Fpid%3DS1517-83822006000300034%26script%3Dsci_art...
BioMed
Central | Full text | Combined use of Amplified Fragment ...
Amplified Fragment Length Polymorphism (AFLP) would scan the entire genome
... Here we evaluate the ability of Amplified Fragment Length Polymorphism (AFLP) ...
www.biomedcentral.com/1471-2334/7/86
Amplified
fragment length polymorphism of Streptococcus suis ...
Amplified fragment length polymorphism of Streptococcus suis strains correlates
with their profile of virulence-associated genes and clinical background ...
jmm.sgmjournals.org/cgi/content/abstract/56/1/102
Chromosome
numbers and pollen stainability of three species of ...
This Article. Right arrow, Full Text. Right arrow, Full Text (PDF). Right arrow,
Submit a response. Right arrow, Alert me when this article is cited ...
www.amjbot.org/cgi/content/abstract/88/4/693
Amplified
Fragment Length Polymorphism Analysis of Campylobacter ...
The high-resolution genotyping method of amplified fragment length polymorphism
(AFLP) analysis was used to study the genetic relationships between ...
aem.asm.org/cgi/content/abstract/66/9/3917
Amplified
Fragment Length Polymorphism (AFLP) Provides Molecular ...
Amplified fragment length polymorphism (AFLP) analysis using 17 primer combinations
was carried out on two species of Caladium (C. bicolor and C. ...
aob.oxfordjournals.org/cgi/content/abstract/84/2/155
Amplified Fragment Length Polymorphism Analysis of Salmonella enterica ... Amplified
fragment length polymorphism (AFLP) is a powerful PCR-based ...
www.springerprotocols.com/.../doi/.../978-1-59745-512-1_8