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Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
Stem Cell Research Techniques and Protocols (1) (2)
(source: Millipore)
Stem cell research has an
enormous potential to transform scientific understanding and ultimately improve the quality of human life. We stand shoulder to shoulder
with our customers, taking the lead among reagent companies in its focus, investment and commitment to stem cell research. Our mission
is to enhance human life and build value by supporting all facets of biomedical research. To support our mission, we have hired scientists
at all levels from labs renowned for their stem cell research and we have increased funding for research and development efforts and
collaborations.
Our collaborations with leading scientists in the stem cell field have led to exclusive licensing agreements with Stem
Cell Sciences, Ltd. for mouse and human ES cell media and with Axordia, Ltd. for ES cell-specific biomarkers. In addition to several
exclusive distribution agreements, we took the first commercial human stem cell license from WARF.
As a result of our investment, we
were the first company to release a complete medium for human
embryonic stem cells. We now offer over 300 stem cell products, created
specifically for human and
rodent, adult and embryonic stem cell research and proven as solutions for use with stem cells. Noteworthy
among our products are the ESGRO? mLIF Media Supplement, the ESGRO Complete? Serum-Free Culture System for Mouse ES Cells, and the
HEScGRO? Medium for Human ES Cell Culture.
Millipore Products for Human ES Research
Millipore¡¯s unique product offering of optimized
media, cell lines, characterization kits and novel, proprietary markers for human embryonic stem cell research comprises an integrated
solution for ...
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Pluripotent Stem Cell Research
Pluripotent stem cells, including Embryonic Germ (EG), Embryonal
Carcinoma (EC) and Embryonic Stem (ES) cells have the capacity to give rise to differentiated progeny representative of all three
...
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HEScGRO Medium is the first commercially available, animal component-free (ACF) medium that has been
successfully tested for human embryonic stem cell culture. No other hES cell medium is ...
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Membrane-based Stem
Cell Culture
We support cell culture with a large family of new and innovative membrane-based products and protocols...
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Millipore provides the largest and most comprehensive range of products for your mouse ES cell culture needs.
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More...
Characterization & Detection Kits
The Alkaline Phosphatase Detection Kit is a specific and sensitive tool for the
phenotypic assessment of ES cell differentiation based upon Alkaline Phosphatase (AP) activity.
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Kits for Pluripotent
Stem Cell Research
The ES Cell 3D Collagen Culture Kit is intended for the investigation of ES cell differentiation and for the formation
of tissue-like structures in a three-dimensional environment in vitro. This kit contains ...
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The
identification of growth factors required for the ex vivo culture of human pluripotent stem cells is critical for stem cell research.
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Markers of Pluripotent Stem Cells & Early Differentiation
In addition to morphological differences, human and
murine pluripotent stem cells differ in their expression of a number of cell surface antigens (stem cell markers).
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Markers
of Developmental Lineages
Following differentiation, pluripotent stem cells give rise to cells of all three embryonic germ layers.
Specialized cells from these germ layers constitute the ...
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Multipotent Stem Cell Research
In addition to pluripotent
stem cells derived from fetal tissues, stem cells have been identified in a diverse range of adult tissues and organs, including neural
stem cells in the brain and hematopoietic....
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Cells for Neural Stem Cell Research
ReNcell immortalized human neural
progenitors and serum-free Media....
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Hematopoietic Stem Cells (HSCs)
Our understanding of bone marrow stem cell
biology has been developed over the last 80 years. The development of this field has led to Hematopoietic Stem Cell (HSC) transplants...
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Angiogenesis, the process of the formation and sprouting of new blood vessels, occurs
during embryonic development and adult life.
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Epithelial Progenitor-Cell-Targeted (PCT) Media
Millipore is proud
to be the distributor of CELLnTEC¡¯s new range of Progenitor Cell Targeted (PCT) epithelial cell culture media. These media have been
proven to:
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Mesenchymal Stem Cells (MSCs)
In addition to Hematopoietic Stem Cells, bone marrow contains several progenitor/stem
cells that not only play an important role in hematopoiesis, but also have the capacity to differentiate into osteoblasts, adipocytes
and chondroblasts. These cells, termed Mesenchymal Stem Cells (MSCs)...
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Analysis of Stem Cell Engraftment Markers
To
determine the utility of pluripotent stem cells for cell transplantation, it is critical to detect stem cell engraftment and to track
their differentiation into specialized cells. The ability to genetically ...
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Rat Neural Stem Cell Expansion Protocol
This
protocol describes the methods used to expand rat neural stem cells and is based on the use of the Rat Neural Stem Cell Expansion
Medium....
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Astrocyte Differentiation Protocol
This protocol utilizes the Astrocyte Differentiation Medium a specially
formulated medium optimized for the preferential differentiation...
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Experimental Outline for Targeting ES Cells
- Step by Step
Plan out a flow chart from day 1 to day 16¨C19.
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EmbryoMax Primary Mouse
Embryo Fibroblasts (PMEF) feeder cells from Millipore are supplied as frozen vials containing...
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Procedure for
Coating Multiwell Plates with Gelatin Solution
Materials and reagents required...
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ES Cell Culture without PMEF Feeder
Cells Protocol
Step by step protocol...
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Feeder-free and Serum-free ES Cell Culture Using ESGRO Complete System Protocol
Step
by step protocol...
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Rescue of ES Cell Lines Using RESGRO Culture Medium
RESGRO Culture Medium has the capacity to
rescue traditional ES cell lines that have started drifting and either generate low percentage chimeras...
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Handling
EmbryoMax Frozen Mouse Embryo Media
EmbryoMax Frozen Mouse Embryo Media are formulated for optimal, consistent performance. These media
are complete, pH adjusted...
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Step by step protocol...
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Protocol for serum-containing medium...
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Step by step protocol...
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Depending upon the cell line and the media used, ES cell colonies are generally ready for picking 5¨C10 days...
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Step by step protocol...
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