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Stable Isotope (or nonradioactive isotope) for Diagnostic Medicine

 

Introduction: Isotopic tracers have been used in medical research for more than fifty years. Radioactive isotopes have been most used because of the high detection efficiencies possible. With increased awareness of the effects of low level radiation and radioactive waste management problems, the need for safe non radioactive tracers has become apparent. Rare stable isotopes of biologically active elements can be used for metabolic and pharmacokinetic studies provided that both sufficient detection sensitivity can be achieved and reliable cost effective instruments can be developed. High resolution optical spectroscopic methods which can determine isotopic ratios with high precision and accuracy are viable for research and clinical use.

 

Stable Isotope Protocols: Sampling and Sample Processing

In this protocol, sediments are not removed from the marsh surface and the .... Stable isotope data are expressed in part per thousand (¡ë) deviation from ...
levin.ucsd.edu/imgs/stable%20isotope%20protocol.doc

 

In-gel stable isotope labeling for relative quantification using ...

In-gel stable isotope labeling for relative quantification using mass spectrometry ... We present a protocol for quantifying changes in the abundance of a ...
www.nature.com/nprot/journal/v1/n1/abs/nprot.2006.7.html

 

Nature Protocols: DNA stable-isotope probing

Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate ...
www.natureprotocols.com/2007/05/24/dna_stableisotope_probing.php

 

Nature Protocols: RNA stable-isotope probing

Recently, the use of stable isotopes ( 13 C) to trace carbon from specific substrates into microbes that assimilate carbon from that substrate has ...
www.natureprotocols.com/2007/05/24/rna_stableisotope_probing.php

 

Mass spectrometry service to assess protein turnover using stable isotope method ...

... protein turnover using stable isotopes 13C leucine and 15N glycine. ... They completed the protocol twice: when the test meals included 3 mg/kg of ...
www.metsol.com/protein_turnover.htm

 

Nature Protocols: A practical recipe for stable isotope labeling in cell culture ...

Search. This journal, All of nature.com. Advanced search ¡¤ Advertisement. This Protocol is listed in the following Categories: ...
www.natureprotocols.com/2007/01/11/a_practical_recipe_for_stable.php

 

Mass spectrometry service to assess glucose metabolism, glucose ...

The primed constant infusion of stable isotope labeled glucose has been extensively ... The following protocol illustrates a method for measuring glucose ...
www.metsol.com/glucose.htm

 

A stable isotope minimal model protocol with oral glucose administration ...

Order from the British Library: A stable isotope minimal model protocol with oral glucose administration.
direct.bl.uk/research/1E/30/RN180557809.html

 

Isotope-coded Affinity Tagging of Proteins Protocol

More recently, Mass Spec (MS) methods based on stable isotope quantitation ... in this protocol, involves the use of isotope-coded affinity tags (ICATs). ...
www.sigmaaldrich.com/.../Protein_Expression/Protocols/Affinity_Tagging_of_Proteins.html

 

A stable isotope minimal model protocol with oral glucose administration ...

A protocol for investigating glucose metabolism whereby stable isotope tracer is given intravenously. after an oral glucose challenge is described. ...
doi.wiley.com/10.1002/rcm.2326

 

A standard protocol for stable isotope analysis of zooplankton in ...

Aug 30, 2007 ... 2007 A standard protocol for stable isotope analysis of zooplankton in aquatic food web research using mass balance correction models. ...
nora.nerc.ac.uk/886/

 

A protocol for preparing subfossil chironomid head capsules for stable isotope analysis ...

also be used as standard protocol for stable isotope. analyses of other elements such as carbon, nitrogen. (Wooller et al. 2007) and hydrogen. The smaller ...
www.springerlink.com/index/a1476j6325828471.pdf

 

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