Liquid chromatography--Mass Spectrometer (GC/MS)
Background:
Liquid chromatography-mass spectrometry (LC-MS) is an analytical
chemistry technique that combines the physical separation capabilities of liquid chromatography (aka HPLC) with the mass analysis
capabilities of mass spectrometry. LC-MS is a powerful technique used for many applications which has very high sensitivity and specificity.
Generally its application is oriented towards the specific detection and potential identification of chemicals in the presence of
other chemicals (in a complex mixture).
1. LC/MS Protocol (created by Shula Levin) --detailed manipulation procedure included
http://www.forumsci.co.il/HPLC/LCMS_ScanMill4.pdf
2. Sterol lipids analysis by Positive ESI Liquid chromatography mass spectrometer
3. Phytohormones--LC/MS Protocol
4. Protocol to extract ribosomal proteins for LC/MS analysis
5. Methods
for full protein sequence mapping: LC-MS preparation
Systematic troubleshooting for LC/MS
References: Short abstracts on LC/MS
techniques.
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics