Hybridization In Situ

 

1. Protocol 1. Nonradioactive in situ hybridization application Manual 

 

2. Protocol 2.  Biotin Labeling and Hybridization in situ protocols and more.

 

3. Protocol 3. FISH: Fluorescence In Situ Hybridization

 

4. Protocol 4. In Situ Hybridization of Mammalian Cells (RNA and Oligonucleotide Probes)

 

5. Protocol 5. In Situ Hybridization in Digoxigenin Labeled Probes

 

6. Anderson Lab. In Situ Hybridization Protocols

 

7. 96 well RNA in situ hybridization protocol

 

8. Protocols for Nanogold-Silver in Situ Hybridization

 

9. In Situ Hybridization with RNA Probes

 

9. In Situ Hybridization in Frozen Sections

 

In Situ Hybridization---the issues

 

Frequently asked questions about in situ hybridization

 

Frequently asked questions about in situ hybridization

 

10. 35S-labeled probe 


11. Anderson Lab In Situ Hybridization Protocols  


12.Autoradiography for mRNA detection in mouse embryo tissue sections 


13. Chick, mouse, and Xenopus two colour whole mount ISH 


14.Chromosome In Situ Hybridization using biotin labeled probes 


15. Chromosome preparation for ISH 


16. Culture and harvesting of human metaphase chromosomes 


17. Denaturation of DNA in ultrathin sections of Lowicryl K4M embedded material 


18. Detection and amplification of FISH signal 


19. Detection of mRNA by in situ hybridization
  

 

 

Fluorescence in situ hybridization (FISH)

 

 
 

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

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