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Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

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Recombinant DNA Techniques (Next Page)

 

Recombinant DNA is a form of synthetic DNA that is engineered through the combination or insertion of one or more DNA strands, thereby combining DNA sequences that would not normally occur together. In terms of genetic modification, recombinant DNA is produced through the addition of relevant DNA into an existing organismal genome, such as the plasmid of bacteria, to code for or alter different traits for a specific purpose, such as immunity. It differs from genetic recombination, in that it does not occur through processes within the cell or ribosome, but is exclusively engineered. Recombinant protein is protein that is derived from recombinant DNA.

 

The Recombinant DNA technique was engineered by Stanley Norman Cohen and Herbert Boyer in 1973. They published their findings in a 1974 paper entitled "Construction of Biologically Functional Bacterial Plasmids in vitro", which described a technique to isolate and amplify genes or DNA segments and insert them into another cell with precision, creating a transgenic bacterium. Recombinant DNA technology was made possible by the discovery of restriction endonucleases by Werner Arber,Daniel Nathans, and Hamilton Smith, for which they received the 1978 Nobel Prize in Medicine.

 

 

A bacterial genetics and recombinant DNA quiz
http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/recomb_DNA.html

 

About Recombinant DNA

http://library.thinkquest.org/04apr/00217/en/products/recdna/about.html

 

Biological Safety: Recombinant DNA Research

http://www.drs.illinois.edu/bss/factsheets/rdna.aspx?tbID=fs

 

Cloning: Dolly Company Used Woman’s DNA
http://news.bbc.co.uk/hi/english/uk/scotland/newsid_816000/816791.stm

 

Goat/Sheep Hybrid
http://news.bbc.co.uk/hi/english/sci/tech/newsid_813000/813466.stm

 

Historical events in recombinant DNA
http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/debate/debate3.htm

 

NIH Guidelines for Recombinant DNA Work (132 pages, in PDF format)

http://oba.od.nih.gov/oba/index.html

 

Pig Clone to Human Transplant
http://news.bbc.co.uk/hi/english/sci/tech/newsid_677000/677637.stm

Protocols for Recombinant DNA
http://www.genome.ou.edu/protocol_book/protocol_index.html

 

Recombinant DNA technology problem set
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/recombinant_dna.html

 

rDNA (Animation)

http://www.present.udel.edu/biotech/rDNA.html

 

Recombinant DNA and Gene Cloning

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html

 

Recombinant DNA (Prof Peter Chantler)

http://www.rvc.ac.uk/review/DNA_1/Index.cfm

 

Recombinant DNA Tutorials (BioG 1103-1104)

http://biog-101-104.bio.cornell.edu/BIOG101_104/tutorials/recomb_DNA.html

 

Recombinant DNA Techniques (Next Page)

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