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Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics
Glucose and Insulin Tolerance Tests (1) (2)
glucose tolerance test protocol
Fast the Animals
Remove
food from the animal cages the night before experiments (aim for 16 hours fasting)
Supplies Needed
Animal restrainers, check if
they are appropriate for the size of animal.
Glucose (50%) 0.2gm/100gm body weight.
IV cannula (22-26 gauge).
Small butterflies.
Warm
water bath.
Plastic, clear tape.
T-connector.
Normal saline for flush (sterile).
1 ml tuberculin syringes.
Blood collection tubes.
Glucometer
and strips.
Alcohol swab.
2x2 gauze.
Filter paper.
Timers.
Excel worksheet to record date, animal group, weight, dose of glucose, volume
, time and route given. Glucose reading at 0 min, 15 min, 30 min, 60 min, 120 min, and 180 min.
Glucose to be given (0.2gm/100gm body
weight)
Procedure
Weigh animal and place in the restrainer.
Warm tail by placing in luke warm water.
Place and secure tail vein
catheter, flush via T-connector.
Give glucose intraperitoneal (IP).
After 15 minutes take 0 minute blood sample for glucose and insulin
investigation via tail.
Take blood samples at 15, 30, 60, 120, and 180 minutes.
Remove IV cannula and press to stop bleeding.
Other
glucose and insulin tolerance test protocols
Glucose Tolerance Test Protocol (Kellylab)
Glucose tolerance test and protocol - The Jackson
Laboratory
Glucose tolerance test and fasting insulin test protocol (Whitelabs)
Glucose tolerance test (Yan Lab)
Simplified Intraperitoneal
Glucose Tolerance Test Procedure
Program & Diabetes Research (Insulin Tolerance Test)
Insulin tolerance test and random fed insulin
test protocol (Whitelabs)
Glucose tolerance Test (GTT) (macdougald Research Lab)
Insulin Tolerance Test Protocol (Kellylab)