Molecular Separation / Molecular  Separation - Affinity chromatography 


Background Information: A major goal in the biochemistry or molecular biology laboratory is the isolation of a specific protein from complex mixtures. Such isolation procedures often involve several fractionation steps with the effectiveness of each step monitored by electrophoresis. Chromatography is a type of fractionation similar to electrophoresis in that proteins separate from each other as they are passed through a matrix. With chromatography, however, solvent flow (by gravity, pressure or capillary action), not an electric field, carries the proteins through the matrix. The separation occurs because proteins interact with various matrices in different ways. Chromatography matrices are designed to exploit the physical and chemical interactions of a protein of interest. For example, acidic proteins will interact with a basic matrix to a greater extent than basic or neutral proteins so a basic matrix can be used to separate acidic proteins (which will interact with the matrix and be retained) from non-acidic proteins (which will flow through the matrix). The acidic proteins retained by the matrix can then be removed or eluted by disrupting their interactions with the matrix. The major limitation of most types of chromatography is the lack of specificity for only one protein, for example a basic matrix willretain all acidic proteins to various extents and not just the specific protein of interest.


Affinity Chromatography C A Handbook
Affinity chromatography separates proteins on the basis of a reversible interaction ... This handbook describes the role of affinity chromatography in the ...


Affinity chromatography - Wikipedia, the free encyclopedia

Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between ...


Affinity Chromatography

The goal of affinity chromatography is to separate all the molecules of a particular specificity from the whole gamut of molecules in a mixture such as a ...


Affinity Chromatography

Restek From the Beginning Phases Phase creation Conducting an analysis ...


Affinity Chromatography Method

Affinity Chromatography Method. Affinity chromatography is designed to purify a particular protein from a mixed sample. Figure 1. Loading affinity column. ...


GE Healthcare Life Sciences - Animation of affinity chromatography

If you have problem viewing affinity chromatography animation in the pop-up window, ... Download the Affinity chromatography animation below GE_Affinity.swf ...


Affinity Chromatography - Sigma

Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand.


Springer Protocols: TOC

Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification ... Dye-Ligand Affinity Chromatography for Protein Separation and Purification ...


Affinity chromatography system for parallel purification of ...

We describe the development and some application results of a simple affinity chromatography system that can be used for the parallel purification of 24 ...


Troubleshooting affinity chromatography
Troubleshooting affinity chromatography. Problem: Tagged protein appears in washes, does not bind to affinity resin. Possible cause ...




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