PCR PRIMER DESIGN AND REACTION OPTIMISATION  

 

Factors Affecting the PCR  

 

Denaturing Temperature and Time

 

Annealing Temperature and Primer Design

 

Primer Length

 

Degenerate Primers

 

Elongation Temperature and Time

 

Reaction Buffer

 

Cycle Number

 

Nested Primer PCR

 

Labelling of PCR products with digoxygenin-11-dUTP

 

Helix Destabilisers / Additives

 

Useful Universal cDNA PCR Primer

 

A simple set of rules for primer sequence design

 

REFERENCES

 
 

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

Free eBooks at Library Online

Cinema Online,Free Movies-(1)

Progresses in Life Science

Free eBooks in biomedicine

Pathway databases

Biological Educational Resources

Textbooks and Lab Manuals

Lab-Manual.Com
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