Chromatin Immunoprecipitation (ChIP) Protocols and Online Resources
What is ChIP? The principle of ChIP is simple: the selective
enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognise a protein or protein modification of interest
can be used to determine the relative abundance of that antigen at one or more locations in the genome.
ChIP consists of the following steps: 1. Isolation of total chromatin (which may require cross-linking to fix the antigen of interest to its chromatin binding site) 2. Fragmentation of the chromatin (to achieve resolution) 3. Immunoprecipitation of the resulting chromatin fragments 4. Analysis of the immunoprecipitated fraction to determine the level of a target DNA sequence (or sequences) relative to its abundance in the input chromatin. The final analysis is typically carried out using PCR or hybridisation-based techniques. A comprehensive review of the procedures and methodologies of ChIP can be found in Allis & Wu (2004).
Chromatin Immunoprecipitation (ChIP) Protocols -- (1) (2)
Protocols - for Chip on Chip
Protocols used in ChIP-on-chip experiments. Listed here are collections of
RenLab protocols as well as protocals requested from other labs. .
www.chiponchip.org/protocol.html
Chromatin Immunoprecipitation (ChIP)
of Protein Complexes: Mapping ...
This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions
of proteins or protein complexes with DNA in vivo ...www.cshprotocols.org/cgi/content/full/2006/23/pdb.prot4560
Epigenome NoE - protocol:
Chromatin Immunoprecipitation (ChIP) Assay
... and Copyright Information ?? Contact Us. ? 2004-2009 Epigenome Network of Excellence
Printed from: http://www.epigenome-noe.net/researchtools/protocol.php.
www.epigenome-noe.net/researchtools/protocol.php?protid=10 Paul's
CHIP - ChIP Protocol for Yeast
Mirmira Lab ChIP Protocol. Updated 12/3/02. Reagents:. ChIP sonication Buffer
(1% .... Resuspend Protein A/G agarose so that it forms a uniform suspension. ...
faculty.virginia.edu/mirmira/resources_files/Protocols/MirmiraLabChIPprotocol.htm
ChIP Protocol - Oxford Gene Technology
Custom Microarray Services ? ChIP Zone ? ChIP Protocol ... DNA:protein complexes immunoprecipitated
using an antibody to the protein of interest ...
www.ogt.co.uk/cms-chip-protocol.htm
Protocols- DNA Protocols > Chromatin Immunoprecipitation
Protocols
ChIP assay protocol with 2 steps: in vivo formaldehyde cross-linking of whole cells and protein-protein and protein-DNA interactions,
...
www.molecularstation.com/protocol-links/DNA-Protocols/Chromatin-Immunoprecipitation-Protocols/
ChIP Protocol for X.laevis Lens1/FoxE3
Enhancer
ChIP protocol for X. laevis Lens1/FoxE3 enhancer Hajime Ogino 10/1/2007 .... (EZ ChIP kit) and incubate at 65¡ãC for overnight
to reverse the DNA-protein ...
faculty.virginia.edu/xtropicalis/chIP_analysis.htm
ChIP Protocol. Wende/Fong/Duncan. Kelly
Lab. Revised May 29, 2007. DAY ONE (for cells): ... Keep at RT until protocol directs to put on ice. ...
kellylab.wustl.edu/protocols/ChIP_Protocol5-29-07.pdf
¨C
Chromatin Immunoprecipitation (ChIP) Assay Protocol
(ChIP) Assay Protocol. Can be prepared in advance and stored at -80o ... ChIP
Protocol. Page 2 of 12. Updated 6/30/06 By Heather N. Witt ...
www.genomecenter.ucdavis.edu/farnham/pdf/FarnhamLabChIP%20Protocol.pdf
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab). This protocol is based upon protocols
from Mark Biggin, Dave Allis and Richard ...
genomecenter.ucdavis.edu/farnham/farnham/protocols/tissues.html
CROSS-LINKING CHROMATIN
IMMUNOPRECIPITATION (X-ChIP) PROTOCOL
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION. (X-ChIP) PROTOCOL. Cross-linking and Cell Harvesting.
1. Start with 2 large dishes when confluent (1x10 ...
www.cosmobio.co.jp/product/kiki/pdf/ACM_chip_protocol.pdf
Day-and-a-half and
two-and-a-half day ChIP
http://blog-di-j.blogspot.com/2007/12/optimized-chip-protocols.html. The blog post will also contain the most
recent version of this protocol. 1.1 Protocol ...
www.jeremiahfaith.com/blog_figs/new_protocol_v1.0.pdf
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics