Microarray Protocols
 
Basic knowledge of microarray.
 
Basic knowledge of microarray 2.
 
Introduction to Microarray.
 
 

MicroArray Procedure

 

(Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall, 8.20.2000)

 

1. Total RNA Isolation from cultured cells.

 

2. DNase Treatment of Total RNA 

 

3. Making the single strand cDNA probe.

 

4. Automated Slide Processor (ASP) Version for hybridization.

 

5. Washing microarrays in ASP.

 

6. Processing of Array slide

 

7. Pre-hybridization of the processed slides (NON-Automated version).

 

8. Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).

 

9.Preparation of Dendrimer Cy3 and Cy5.

 

10. Washing unbound probe from glass array (NON-Automated version).

 

11. Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).

 

12. Washing unbound dendrimer from glass array(NON-Automated version).

 

Microarray Dababases

 

Troubleshooting

 

Other Microarray Protocols (1)

Other Microarray Protocols (2)
                                                                                            
 

Microarray Databases

 

Public microarray database

Public microarray database is a public repository of microarray data. It often accompanies with some data analysis and/or visualization tools. ...
ihome.cuhk.edu.hk/~b400559/arraysoft_public.html

Stanford MicroArray Database

SMD, the Stanford MicroArray Database, stores raw and normalized data from microarray experiments, and provides data retrieval, analysis and visualization ...
genome-www5.stanford.edu/

Microarray Database Gateway

Tools for managing, accessing, and analyzing cDNA microarray data generated by the NCI Div. of Clinical Sciences µArray Center - from the BioInformatics and ...
nciarray.nci.nih.gov/

yMGV - yeast Microarray Global Viewer

July 29th 2003: 6 new S. cerevisiae datasets added Older news. Last database update: 2003-09-07 Last interface update: 2003-09-16 The yMGV team.
transcriptome.ens.fr/ymgv/

Yale Microarray Database (YMD)

The Yale Microarray Database Project is a collaborative effort between several laboratories and centers to meet the urgent need to: ...
www.med.yale.edu/microarray/

UNC MicroArray Database

If you have further questions regarding access, please e-mail the UNC-Chapel Hill Microarray Database curators at: UNC MD curators ...
https://genome.unc.edu/

MUSC Microarray Database

Full access to the MUSC DNA Microarray Project Management System and the MUSC DNA Microarray Database is free but requires that you register as a user. ...
proteogenomics.musc.edu/ma/musc_madb.php?page=home&act=manage

ArrayExpress Home

Database of gene expression and other microarray data at the European Bioinformatics Institute (EBI), located at the Wellcome Trust Foundation in Cambridge, ...
www.ebi.ac.uk/arrayexpress/

SGMD Home

The soybean genomics and microarray database (SGMD) was established in 1999 to serve as a sequence and microarray database for the Soybean Genomics and ...
psi081.ba.ars.usda.gov/SGMD/default.htm

Many Microbe Microarrays Database

M3D is a resource for analyzing and retrieving Affymetrix expression data for microbes (currently E. coli, S. cerevisiae, and S. oneidensis).
m3d.bu.edu/

Microarray database/ Laboratory Information Management System ...

A closely related species of database software is public microarray database, a public repository of microarray data. Please refer to My Microarray Software ...
ihome.cuhk.edu.hk/~b400559/arraysoft_database.html

Microarray Database - University of Tennessee

UTMD, the Microarray Database - University of Tennessee, stores raw and normalized data from microarray experiments, and provides data retrieval, ...
genome.ws.utk.edu/

MGED - Microarray and Gene Expression Data Home

The Microarray and Gene Expression Data (MGED) Society is an international organization of bi

UHN Human CpG Island Microarray Database

Welcome to the UHN Microarray Centre's CpG Island Database! Here you will find all the information you need to get started with analysing your results from ...
data.microarrays.ca/cpg/

microarrays.org

AMAD: Another Microarray Database. (Jan 1, 2000 - version 1.01) This is a simple yet very effective flat file database designed specifically for microarray ...
derisilab.ucsf.edu/microarray/software.html

 

 

 

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