Other Online Microarray Protocols -- (1)  (2)  (3)
 
General Microarray Protocols  

Pat Brown's Laboratory ­ Stanford

John Quackenbush ­ TIGR

National Human Genome Research Institute (NHGRI)

The Institute for Genomic Research (TIGR)

Microarray Core Facility (Baylor College of Medicine)

Complete protocol for epoxy slides (QUANTIFOIL)

Complete protocol for aldehyde slides (QUANTIFOIL)

Complete protocol for amino slides (QUANTIFOIL)

DNA Amplification and Purification

Preparation of DNA Samples (Brown Laboratory ­ Stanford)

National Human Genome Research Institute (NHGRI)

Template Miniprepping (TIGR)

PCR Purification and Storage (TIGR)

Montage PCR Cleanup Filter Plates (Millipore)

Slide Array Preparation & Printing

Poly-l-Lysine coating of slides (Brown Laboratory-Stanford)

Post-Processing Arrays (Brown Laboratory-Stanford)

National Human Genome Research Institute (NHGRI)

Printing in DMSO (TIGR)

Slide array preparation and printing (QUANTIFOIL)

RNA Isolation

Fast Track mRNA Isolation (Brown Laboratory ­ Stanford)

Preparation of Total RNA from Blood (Brown Laboratory-Stanford)

RNA Extraction (NHGRI)

Poly A+ Isolation from Total RNA (TIGR)

FastTrack?2.0 mRNA Isolation Kit (Invitrogen)

Micro-FastTrack?2.0 mRNA Isolation Kit (Invitrogen)

mRNA Catcher?(Invitrogen)

TRIzol?Reagents (Invitrogen)

Technical Article (Ambion, Inc.)

Total RNA isolation kits and reagents (Ambion, Inc.)

mRNA isolation kits and reagents (Ambion, Inc.)

ArrayIt Micro-Total RNA Extraction Kit (Telechem)

RNA Amplification

aRNA Amplification - Ambion Method (Brown Laboratory ­ Stanford)

aRNA Amplification - Wang Method (Brown Laboratory ­ Stanford)

aRNA Amplification (Brown Laboratory ­ Stanford)

Modified Eberwine RNA Amplification

Amino Allyl MessageAmp?aRNA Kit (Ambion, Inc.)

MessageAmp?II aRNA Amplification Kit (Ambion, Inc.)

BDSmart mRNA Amplfication Kit (BD Biosciences Clontech)

ArrayIt MiniAmp mRNA Amplification Kit (Telechem)

Probe Labeling

OU Microarray Core Facility (University of Oklahoma)

Saied A. Jaradat, Ph.D., Tetsuya S. Tanaka, Ph.D. and Yulan Piao, M.D ­ (National Institute of Aging)

Labeling Yeast RNA (Brown Laboratory ­ Stanford)

Labeling Human RNA (Brown Laboratory-Stanford)

LabelingE.Coli RNA (Brown Laboratory-Stanford)

Amino-Allyl Reverse Transcription (Brown Laboratory-Stanford)

Revised Amino-Allyl Labeling (Brown Laboratory-Stanford)

Labeling by Reverse Transcription (NHGRI)

Amino-allyl Dye Coupling Protocol

Reverse Transcription/Labeling RNA

Modified Eberwine RNA Amplification

Amino Allyl Labeling (TIGR)

Amino Allyl cDNA Labeling Kit (Ambion, Inc.)

FairPlay?II Microarray Labeling Kit (Stratagene)

LabelStar Array Kit (Qiagen)

ArrayIt Indirect Amino Allyl Fluorescent Labeling Kit (Telechem)

ULS?labeling kits for DNA microarray applications (Kreatech)

Microarray Target Purification Kit (Roche Biochemicals)

Label IT?µArray?Cy?/Cy? Labeling Kit (Mirus Bio Corp.)

Hybridization & Washing

Array Hybridization-General/Yeast (Brown Laboratory-Stanford)

Array Hybridization-Human (Brown Laboratory-Stanford)

Hybridization (NHGRI)

Labeled Probe Hybridization (TIGR)

Hybridization/Washing Glass Slide Arrays (QUANTIFOIL)

Array Washing Protocol (QUANTIFOIL)

Affymetrix GeneChips?Protocols

W.M. Keck Facility (Yale University)

 

 

 
 

Microarray Protocols

 

Basic knowledge of microarray.

 

Basic knowledge of microarray 2.

 

Introduction to Microarray.

 

A Mediabook of Microarrays

 

Protein chips 

 

MicroArray Procedure

(Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall, 8.20.2000)

 

1. Total RNA Isolation from cultured cells.

 

2. DNase Treatment of Total RNA

 

3. Making the single strand cDNA probe.

 

4. Automated Slide Processor (ASP) Version for hybridization.

 

5. Washing microarrays in ASP.

 

6.Processing of Array slide

 

7. Pre-hybridization of the processed slides (NON-Automated version).

 

8. Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).

 

9. Preparation of Dendrimer Cy3 and Cy5.

 

10. Washing unbound probe from glass array (NON-Automated version).

 

11. Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).

 

12.Washing unbound dendrimer from glass array(NON-Automated version).

 

Microarray Dababases

 

Troubleshooting

 

Other Microarray Protocols (1)

 

Other Microarray Protocols (2)

 

Other Microarray Protocols (3)

Web Guider

Ch 1.General Lab Techniques

Ch 2.Molecular Separation

Ch 3.DNA and RNA

Ch 4.Genetics

Ch 5.PCR Serials

Ch 6.Protein

Ch 7.DNA Protein Interactions

Ch 8.Immunohistoch / immunology

Ch 9.Cellular Biology

Ch 10.GC/MS, NMR and Proteomics

Ch 11.Animal Experiments

Ch 12.Worm: C. Elegans

Ch 13.HPLC and TLC

Ch 14.Buffers formats in Lab.

Ch 15.Other Resources

Free eBooks at Library Online

Cinema Online,Free Movies-(1)

Progresses in Life Science

Free eBooks in biomedicine

Pathway databases

Biological Educational Resources

Textbooks and Lab Manuals

Lab-Manual.Com
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