Cholesterol
Cholesterol is run underivatized or with TMS..
Samples: Unlabeled cholesterol, and cholesterol extracted from a saponified
fraction.
GC/MS:
GC column: HPUltra1, length-12m, 250mM, programmed at a constant He flow of 1ml/min.
Inlet temp. 260oC,
split mode.
Oven temperature programmed as follows: 170oC for 2 min, then a 35oC/min ramp to 278oC and hold for 6min. Cholesterol RT
5.85 min
Relevant ion cluster for SIM analysis, m/z 386.
Web Guider
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