3M ammonium acetate

• Dissolve 231.24g of ammonium acetate in 1 liter dH₂O.

10X TBE

• 100 gm/L     (0.89M Tris) MW 121.14
• 55 gm/L     (0.89M Boric acid) MW 61.83
• 40ml of 0.5M EDTA disodium

Adjust pH to 8.0 with 1:10 diluted HCl.

 

20X SSC

• 3M NaCl
• 0.3M sodium citrate

100X Denhardt's Solution

• 2% bovine serum albumin
• 2% polyvinylpyrrolidone [Sigma]
• 2% ficoll

Denaturing Solution

• 0.5M NaOH
• 1.0M NaCl

DNA Loading Dye

• 15% ficoll [F-4375 Sigma]
• 10% glycerol
• 0.05% bromophenol blue
• 1X TBE buffer

Weigh 15g of ficoll and add 50-100mg of bromophenol blue, then add 10ml of glycerol and 90ml of 1X TBE.

Place this mixture on a hot plate with a magnetic stirrer and allow to dissolve and make up to 100ml with 1X TBE.

 

DNA Lysis Buffer

• 100mM NaCl
• 10mM Tris-HCl pH 8.0
• 25mM EDTA (disodium salt)
• 0.5% of sodium dodecyl sulphate
• 0.1mg/ml Proteinase K

Ethidium Bromide

• 10mg of ethidium bromide powder
• 1ml of dH₂O

Neutralizing Solution

• 1.5M Tris (pH 8.0)
• 1.0M NaCl