Table of Contents.
** Carbodiimides as fixatives
** Carnoy & alcoholic fixatives
** Perfusion fixative for electron microscopy
** Fixation of frozen sections.
** Non-formaldehyde commercial fixatives
** Glutaraldehyde and immunohistochemistry
** Isopentane: alternative names
** Lidocaine in perfusion fixation
** Michel's fluid for transporting cells or specimens
** Microwave ovens: Advice for new users
** Paraformaldehyde: why won't it dissolve?
** Saccomano's fixative
** Zinc-containing fixatives: What has been published?
** Alternatives to mercury-containing fixatives
PROCESSING, DECALCIFYING, EMBEDDING
** Solvent to
replace xylene AND alcohols
** 2-butoxyethanol ("Clereum") dehydrating or clearing agent
** Decalcification: Acid or EDTA?
** Testing for completeness of decalcification
** Fatty specimens: Processing into paraffin.
** Polymethyl methacrylate embedding for bone
** Mold release spray
** Paraffin processing of skin
** Cryoprotection of
specimens
** Cutting sections of toe or finger nails
** Paraffin wax:
crystals, additives and cutting
** Xylene substitutes: what are they?
** Test for water in used absolute alcohol
** Molecular sieves for making anhydrous solvent
SECTIONING,
SLIDE ADHESIVES, MOUNTING
** Sections coming off slides. Which adhesive?
** Apathy's mounting medium and variants
** Silanized (APES or TES or positively charged) slides
** Polishing undecalcified bone sections.
** Polylysine-coated slides
** Wrinkles in plastic sections
** Wrinkles in paraffin sections containing cartilage
** Thick paraffin sections
** Sectioning plastic-embedded specimens
** Iodine
for removing mercury deposits
** Labeling slides
** Sectioning plant material:
some hints.
STAINING METHODS, HISTOCHEMISTRY
** Making aldehyde-fuchsine
** Phosphatases
in decalcified, embedded tissue.
** Congo red for amyloid
** Cartilage staining
with safranine
** Stain for Chlamydia (Castaneda's method).
** Which staining
method for copper is best?
** Diastase (amylase) control for glycogen
** Evans
blue, trypan blue and eosin as tracers.
** Gallyas' stain
** Gram staining
of sections (Brown & Hopps method).
** Oxidants for hematoxylin
** McFaydean's
stain for anthrax bacilli
** Microglia with Griffonia lectin.
** Picro-sirius
red staining
** Iron hematoxylin: ripening not needed.
** Enzyme histochemistry
on cell cultures
** Malachite green in stain for Cryptosporidium
** Confusing
dye names (lissamine fast red as an example)
** Mayer's and Gill's hematoxylins
** Effects
of pH on staining by dyes
** Histochemical stain for arsenic
** Giemsa staining
of blood smears: several hints
** Automated H & E staining problems
** Verhoeff's
stain for myelin and elastin
** Acridine orange method for DNA and RNA
** Quickly
finding something in a newly cut section
** Fluorescent lectins: general method
** Methyl
blue and methylene blue
** Paraffin or frozen sections for immunohistochemistry
** Inhibiting
endogenous peroxidase
** Using mouse primary antibodies on mouse tissues
** Antigen
retrieval: A patented or copyright phrase?
** p53 protein
** Prevention
of fluorescence fading
** Background in immunostained cartilage
** Endogenous
biotin in mast cells?
** Disposal of used diaminobenzidine (DAB) solutions
** Dilution
of concentrated acids: formula etc.
** Disposal of waste from "special stains"
** Magnification
of a photomicrograph
** Can a method be both published and patented?
** Books
and articles about artifacts in histology
** How dangerous is picric acid?
** Which
color print film for photomicrography?
Web Guider
Ch 8.Immunohistoch / immunology
Ch 10.GC/MS, NMR and Proteomics